Bio-Rad Negative Stains - Manual (Page 4)

The Copper Stain can be ordered separately, as catalog number 161-
0471. The Copper Destain, a 10x Tris/Glycine buffer, can be reordered
in a 1 liter bottle as catalog number 161-0734.
The Copper Staining procedure is recommended for mini-gels,
especially those of 1.0 mm thickness or less. The quantities included
with this kit can be used to stain 25 mini-gels, using 50 ml Copper Stain
for each gel, or 2 full-size gels, using about 600 ml stain for each gel.
1.3 Materials Required But Not Supplied
Graduated cylinders
Distilled, deionized water
Staining and rinsing containers. To optimize the number of gels
stained per kit, small staining containers, such as the lid of a pipet tip
box, should be used for staining mini-gels.
1.4 Safety Considerations
Eye protection and gloves should be worn while handling this
product.
1.5 Disposal Methods
Laws governing disposal of laboratory chemicals vary by region.
Check local laws for disposal of Copper Stain.
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Fig. 1. Mini-PROTEAN ® II 12% ready gel stained with Copper Stain. Bio-
Rad's LMW SDS-PAGE standards were diluted and electrophoresed at 200 V
for 30 minutes. Standards were serially diluted from 500 ng of each protein per
well to 3.91 ng of each protein per well. The gel was subsequently stained with
Copper Stain.
The gel can be stored in water for several weeks without loss of
resolution of the protein bands.
1.2 Product Description
The Copper Stain & Destain Kit, catalog number 161-0470, contains:
125 ml Copper Stain, 10x
125 ml Copper Destain, 10x
Laminated instruction card
Complete instruction manual
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