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Bio-Rad Silver Stains User Manual

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9.3 Nucleic Acids

12. Berry, M. J. and Samuel, C. E., “Detection of Subnanogram Amounts of RNA in

Polyacrylamide Gels in Presence and Absence of Protein by Staining with Silver,”
Anal. Biochem., 124, 180 (1982).

13. McNeilage, L. J. and Whittingham, S., “Use of Bio-Rad Silver Stain to Identify Gel

Purified RNA Components of Small Nuclear Ribonucleoprotein Antigens,” J.
Immunol. Methods
, 66, 253 (1984).

14. Goldman, D. and Merril, C. R., “Silver Staining of DNA in Polyacrylamide Gels:

Linearity and Effect of Fragment Size,” Electrophoresis, 3, 24 (1982).

15. Beidler, J. L., et al., “Ultrasensitive Staining of Nucleic Acids with Silver,” Anal.

Biochem., 126, 374 ( 1982).

9.4 Isoelectric Focusing

16. Mehat, P. D. and Patrick, B. A., “Detection of Oligoclonal Bands in Unconcentrated

CSF: Isoelectric Focusing and Silver Staining,” Neurology, 33, 1365 (1983).

17. Confavreux, C., et al., “Silver Stain After Isoelectric Focusing of Unconcentrated

Cerebrospinal Fluid: Visualization of Total Protein and Direct Immunofixation of
Immunoglobulin G,” Electrophoresis, 3, 206 ( 1982)

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Section 9
References

9.1 General

1.

Merril, C. R., Goldman, D., Sedman, S. A. and Ebert, M. H., “Ultrasensitive Stain for
Proteins l

n

Polyacrylamide Gels Shows Regional Variation in Cerebrospinal Fluid

Proteins,” Science, 211, 1437 (1981).

2.

Laemmli, U. K., “Cleavage of Structural Proteins During the Assembly of the Head
of Bacteriophage T4", Nature, 227, 680 (1970).

3. Switzer, R. C., Merril, C. R. and Shifrin, S., “A Highly Sensitive Silver Stain for

Detecting Proteins and Peptides in Polyacrylamide Gels,” Anal. Biochem., 98, 231
(1979).

4.

O’Farrell, P. H., “High Resolution Two-Dimensional Electrophoresis of Proteins,” J.
Biol. Chem.
, 250, 4007 (1975).

9.2 Proteins

5. Merril, C. R., Goldman, D., Sedman, S. A. and Ebert, M. H., “Ultrasensitive Stain for

Proteins in Polyacrylamide Gels Shows Regional Variation in Cerebrospinal Fluid
Proteins,” Science, 211, 1437 (1981).

6. Merril, C. R. and Goldman, D., “Quantitative Two-Dimensional Protein

Electrophoresis for Studies of Iubom Errors of Metabolism,” Clin. Chem., 28, No. 4
(1982), (reference on effect of recycling on image intensification).

7. Merril, C. R., Goldman, D. and Van Keuren, M. L., “Simplified Silver Protein

Detection and Image Enhancement Methods in Polyacrylamide Gels,”
Electrophoresis, 3, 17 (1982).

8.

Merril, C. R., Goldman, D. and Van Keuren, M. L., “Gel Protein Stains: Silver Stain,”
Methods in Enzymology, 104, 441 (1984).

9. Merril,

et al., “Molecular Probes for Human Genetic Diseases by Two- Dimensional

Electrophoresis and Silver Stain,” in: Electrophoresis '82, p. 327,Walter Degruyter,
publisher.

10. Merril, C. R. and Goldman, D., “Detection of Polypeptides in Two-Dimensional Gels

Using Silver Stain." in: Two-Dimensional Gel Electrophoresis of Proteins (Celis, J.
E. and Bravo, R., eds.), Academic Press, N.Y, 93 (1984).

11. Dzandu, L. K., Deh, M. E., Barratt, D. L. and Wise, G. E., “Detection of Erythrocyte

Membrane Proteins, Sialoglycoproteins and Lipids in the Same Polyacrylamide Gel
Using a Double-Staining Technique, Proc. Nat. Acad. Sci. USA, 81, 1733 (1984).

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