Bio-Rad Silver Stains User Manual
Page 2
Table of Contents
Section 1
Introduction...............................................................1
Section 2
Silver Stain Kit Components ...................................1
Section 3
Important Procedural Information.........................1
3.1
Water Purity .........................................................................1
3.2
Gel Handling .......................................................................2
3.3
Temperature .........................................................................2
3.4
Convenient Stopping Point ..................................................2
3.5
Band Development ..............................................................2
3.6
Destaining............................................................................2
3.7
Storage of Stained Gels .......................................................3
3.8
Photographing Gels .............................................................3
Section 4
Reagent Preparation.................................................4
Section 5
Protocols ....................................................................5
Section 6
Additional Applications............................................7
6.1
Nucleic Acid Staining..........................................................7
6.2
Silver Staining after Coomassie Blue R-250 Staining.........7
6.3
Silver Staining Electrofocusing Slab Gels...........................7
Section 7
Troubleshooting Guide .............................................7
Section 8
Ordering Information...............................................9
Section 9
References................................................................10
9.1
General ..............................................................................10
9.2
Proteins ..............................................................................10
9.3
Nucleic Acids ....................................................................11
9.4
Isoelectric Focusing...........................................................11
LIT34B 6/17/98 2:04 PM Page i