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Bio-Rad Silver Stains User Manual

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Table of Contents

Section 1

Introduction...............................................................1

Section 2

Silver Stain Kit Components ...................................1

Section 3

Important Procedural Information.........................1

3.1

Water Purity .........................................................................1

3.2

Gel Handling .......................................................................2

3.3

Temperature .........................................................................2

3.4

Convenient Stopping Point ..................................................2

3.5

Band Development ..............................................................2

3.6

Destaining............................................................................2

3.7

Storage of Stained Gels .......................................................3

3.8

Photographing Gels .............................................................3

Section 4

Reagent Preparation.................................................4

Section 5

Protocols ....................................................................5

Section 6

Additional Applications............................................7

6.1

Nucleic Acid Staining..........................................................7

6.2

Silver Staining after Coomassie Blue R-250 Staining.........7

6.3

Silver Staining Electrofocusing Slab Gels...........................7

Section 7

Troubleshooting Guide .............................................7

Section 8

Ordering Information...............................................9

Section 9

References................................................................10

9.1

General ..............................................................................10

9.2

Proteins ..............................................................................10

9.3

Nucleic Acids ....................................................................11

9.4

Isoelectric Focusing...........................................................11

LIT34B 6/17/98 2:04 PM Page i