Bio-Rad GS-900™ Calibrated Densitometer - Manual

QC Colloidal Coomassie Stain
Ordering Information
Catalog #
Product
161-0803 QC Colloidal Coomassie Stain, 1 L
Introduction
Bio-Rad’s QC colloidal Coomassie stain is a ready-to-use single-bottle protein stain that does not require the
mixing of any components or addition of any alcohols. It is a special formulation of Coomassie G-250 that
provides maximum sensitivity with low background for a wide variety of acrylamide gel chemistries. The QC
colloidal Coomassie stain can reliably detect BSA in amounts down to 3 ng.
The QC colloidal Coomassie stain does not contain any methanol or acetic acid, which must be disposed of as
hazardous waste.
Kit Contents
Kit contains 1 L of QC colloidal Coomassie stain, which is sufficient to stain 10 Criterion™ gels or 20
Mini-PROTEAN® gels.
Storage Conditions
The QC colloidal Coomassie stain should be stored and used at room temperature. Do not freeze or refrigerate
the stain.
User-Supplied Materials
• Deionized water
• Shallow tray with cover for gel staining and destaining
• Ethanol and acetic acid, if gel fixation is desired
Staining Protocol
This protocol provides the maximal sensitivity while maintaining low background levels and provides the most
consistent and robust results. This protocol allows detection of amounts down to 3 ng of BSA.
Gel Size Fixing Solution*,
ml
QC Colloidal
Coomassie Stain, ml
Water Washes,
ml
Criterion 100 100 100
Mini-PROTEAN 50 50 50
* 40% ethanol, 10% acetic acid
Gel Fixation
Fixation is recommended for maximum sensitivity and staining of low molecular weight proteins <20 kDa.
• Prepare fixing solution (40% ethanol, 10% acetic acid)
• Remove gel from cassette and rinse in a shallow staining tray with deionized water