Bio-Rad PROTEAN® Plus Hinged Spacer Plates and Combs User Manual
Page 2

Table of Contents
Page
Section 1 Introduction..........................................................................................1
1.1 Specifications ....................................................................................................2
Section 2 Pouring a Linear Gradient Gel ...........................................................2
2.1
Calculating the Chamber Volumes ....................................................................2
2.2
Pouring the Gel from the Top ............................................................................2
2.3
Pouring the Gel from the Bottom.......................................................................3
Section 3 Pouring an Exponential Gradient Gel ................................................4
3.1
Concave Exponential Gradient Gel....................................................................4
3.2
Convex Exponential Gradient Gel .....................................................................5
Section 4 Pouring Two Gradient Gels.................................................................5
Section 5 Pouring Multiple Gradient Gels in a Gel Casting Chamber .............6
Section 6 Gradient Former Care and Maintenance...........................................7
Section 7 Theory of Linear and Exponential Gradient Gels .............................7
7.1
Linear Gradients................................................................................................7
7.2
Exponential Gradients .......................................................................................7
Section 8 Protocols .............................................................................................10
8.1
Preparation of Stock Solutions for Laemmli SDS-PAGE Slab Gels ................10
8.2
Calculating the Volume of the Gel...................................................................11
8.3
Gel Preparation................................................................................................12
8.4
Linear Gradient Gels .......................................................................................13
8.5
Exponential Gradient Gels...............................................................................14
Section 9 Equipment and Accessories...............................................................15
9.1
Model 385 and Model 395 Gradient Former and Accessories .........................15
9.2
Additional Required Equipment ......................................................................15
9.3
PROTEAN
®
II xi and Mini-PROTEAN
®
II
Vertical Electrophoresis Cells and Accessories ...............................................15
9.4
Electrophoresis Chemicals...............................................................................17