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1 specifications – Bio-Rad Mini-PROTEAN® Tetra Handcast Systems User Manual

Page 3

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Section 1
Introduction

The Model 485 Gradient Former is primarily used to construct reproducible linear and

exponential polyacrylamide gradient gels. It has a 175 ml capacity, making it ideal for
preparation of up to 12 identical Mini-PROTEAN

®

3 gradient gels. It can also be used to

prepare PROTEAN II gels.

The mixing (labeled "light") and reservoir (labeled "heavy") chambers have identical

dimensions. The outlet from the mixing chamber leads into a multi-casting chamber. The
exponential piston is available as an accessory; it is used to fix the volume in the mixing
chamber to produce concave or convex exponential gradients.

Figure 1.1 Model 485 Gradient Former, major parts

1.1 Specifications

Overall size

10 cm x 10 cm x 16 cm (W x L x H)

Weight

500 g

Capacity

40–175 ml

Materials of construction

Body

Machined from an acrylic block

Valve stem

Delrin

®

Stopcock

Plastic

Piston

Delrin

Tubing

Tygon

®

1/8” ID

1

Exponential piston

Mixing chamber

(light)

Reservoir chamber

(heavy)

Luer taper coupling

Valve stem

Y-connector

Stopcock

Tubing

Multi-casting

Chamber