1 specifications – Bio-Rad Mini-PROTEAN® Tetra Handcast Systems User Manual
Page 3
Section 1
Introduction
The Model 485 Gradient Former is primarily used to construct reproducible linear and
exponential polyacrylamide gradient gels. It has a 175 ml capacity, making it ideal for
preparation of up to 12 identical Mini-PROTEAN
®
3 gradient gels. It can also be used to
prepare PROTEAN II gels.
The mixing (labeled "light") and reservoir (labeled "heavy") chambers have identical
dimensions. The outlet from the mixing chamber leads into a multi-casting chamber. The
exponential piston is available as an accessory; it is used to fix the volume in the mixing
chamber to produce concave or convex exponential gradients.
Figure 1.1 Model 485 Gradient Former, major parts
1.1 Specifications
Overall size
10 cm x 10 cm x 16 cm (W x L x H)
Weight
500 g
Capacity
40–175 ml
Materials of construction
Body
Machined from an acrylic block
Valve stem
Delrin
®
Stopcock
Plastic
Piston
Delrin
Tubing
Tygon
®
1/8” ID
1
Exponential piston
Mixing chamber
(light)
Reservoir chamber
(heavy)
Luer taper coupling
Valve stem
Y-connector
Stopcock
Tubing
Multi-casting
Chamber
- TransFectin™ Lipid Reagent (2 pages)
- Gene Pulser MXcell™ Electroporation System (19 pages)
- Gene Pulser MXcell™ Electroporation System (66 pages)
- Gene Pulser Xcell™ Electroporation Systems (83 pages)
- Gene Pulser® Electroporation Buffer (2 pages)
- MicroPulser™ Electroporator (31 pages)
- Helios® Gene Gun System (52 pages)
- PDS-1000 / He™ and Hepta™ Systems (51 pages)
- TGX™ FastCast™ Acrylamide Solutions (2 pages)
- Criterion™ TGX Stain-Free™ Precast Gels (16 pages)
- Image Lab™ Software (212 pages)
- Gel Doc™ EZ System (22 pages)
- Criterion Stain Free™ Tris-HCl Gels (96 pages)
- Mini-PROTEAN® TGX™ Precast Gels (52 pages)
- ChemiDoc™ XRS+ System (42 pages)
- ChemiDoc™ XRS+ System (4 pages)
- ChemiDoc™ XRS+ System (50 pages)
- GS-800™ Calibrated Densitometer (444 pages)
- ChemiDoc™ MP System (8 pages)
- Image Lab™ Software (236 pages)
- Criterion™ TGX™ Precast Gels (60 pages)
- Criterion™ Cell (13 pages)
- Image Lab™ Software (260 pages)
- Criterion™ XT Bis-Tris Precast Gels (26 pages)
- 2-D Electrophoresis Workflow (14 pages)
- Fluorescent Protein Stains (27 pages)
- 2-D Electrophoresis Workflow (22 pages)
- 2-D Electrophoresis Workflow (20 pages)
- PROTEAN® i12™ IEF System (4 pages)
- ReadyPrep™ 2-D Starter Kit (28 pages)
- EXQuest Spot Cutter (81 pages)
- Ready Gel® Zymogram Precast Gels (46 pages)
- Mini-PROTEAN 2-D Electrophoresis Cell (44 pages)
- Precision Plus Protein™ Prestained Standards (3 pages)
- Precision Plus Protein™ Unstained Standards (16 pages)
- Prestained SDS-PAGE Standards (3 pages)
- Unstained SDS-PAGE Standards (3 pages)
- Silver Stains (20 pages)
- Biotinylated Standards (3 pages)
- Biotinylated Standards (11 pages)
- IEF and 2-D Standards (3 pages)
- Mini-PROTEAN 2-D Electrophoresis Cell (20 pages)
- Mini-PROTEAN 3 Multi-Casting Chamber (10 pages)
- PROTEAN® Plus Hinged Spacer Plates and Combs (21 pages)