LaMotte SAB/SAB - Manual (Page 2)

LaMotte BioPaddles™ TECH DOCUMENT
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Email: [email protected]
For in vitro diagnostic use only. This product should be used only by adequately trained personnel with knowledge of microbiological techniques in the laboratory.
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LaMotte_BioPaddles_SAB_SAB 9.12
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STORAGE / EXPIRATION
Store tightly sealed BioPaddles™ in a cool, dry location. Shield from direct sunlight. Store BioPaddles™ at room
temperature (65 - 77°F/18 - 25°C). Avoid sudden temperature changes. Temperature fluctuations may result in
condensation settling at the bottom of the vial. This will not affect the culture properties, but could reduce the shelf-life or
cause the agar to separate from the plastic paddle support. Do not refrigerate. Refrigeration may result in water
condensation. Avoid freezing. If freezing occurs, thaw for 3-6 hours in the refrigerator (40°F; 4.4°C). Freezing can promote
excess water loss and variation in media surface due to crystal formation.
Refer to Best Before End date (See: BBE stamped on vial). Discard if paddle agar appears oxidized and darker than the
expected color or if contaminants appear. The expiration date is based on medium in an intact container that is stored as
directed.
SAMPLING
Liquids: Twist to remove paddle from vial. Fill vial to 40 mL fill line with the liquid
to be sampled. The 40 mL volume can be used to calculate Total Viable Count
(TVC) and/or Total Colony Count (TCC). Replace paddle. Allow a contact time of
15 seconds. Remove the paddle. Empty the vial. Replace the paddle in the vial.
Surfaces: Twist to remove paddle from vial. Allow the paddle surface (10 cm
2 ) to come into physical contact with the test
surface. Recovery rate is about 50%. To ensure an accurate recovery, gently sweep (or touch) the paddle to cover a 20 cm 2
area. Replace paddle in vial.
INCUBATION
Temperature Minimum Period Optimal Period
35°C (bacteria) 72 hours 5-7 days
20-25°C (fungi) 5 days 7 days
COLONY MEASURING
Each BioPaddles™ paddle has molded media attachment points that are 4mm
in length (point-to-point). This feature provides a useful guidepost to estimating
nearby colony size.