LaMotte SAB/SAB - Manual (Page 10)

LaMotte BioPaddles™ TECH DOCUMENT
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For in vitro diagnostic use only. This product should be used only by adequately trained personnel with knowledge of microbiological techniques in the laboratory.
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LaMotte_BioPaddles_SAB_SAB 9.12
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DISPOSAL
Twist to remove paddle from vial. Fill vial to 40 mL fill line with 1:9 dilution of household bleach (5.25% sodium hypochlorite).
Replace paddle in vial. Allow 15 minute contact time. Remove paddle. Discard bleach solution. Replace paddle in vial and
dispose. Alternatively, loosen cap and microwave for 30 seconds, autoclave, or incinerate.
GLOSSARY:
Catalase Test Catalase enzyme will react with hydrogen peroxide to produce oxygen if the bacteria is
catalase positive.
Lactose Test Lactose positive bacteria can ferment available lactose in the agar producing an acid which
lowers the pH. Lactose negative bacteria are non-fermenting.
Indole Test Biochemical test to determine the ability of an organism to split indole from the amino acid
tryptophan. P. vulgaris is indole positive while P. mirabilis is indole negative.
Oxidase Test Oxidase positive bacteria contain cytochrome c oxidase which will turn an indicator dark blue. In
contact with oxidase negative bacteria, the indicator will remain colorless.
Urease Test Bacteria containing urease will hydrolyze urea to ammonia and carbon dioxide
causing an alkaline environment which changes the color of a pH indicator from yellow to fuchsia.
β-D-Glucoronidase The presence of E. coli is determined when both β-D-Glucoronidase and Indole
Reaction are positive, and the organism is gram negative.
Gram Staining A method for differentiating bacteria into two groups – gram positive and gram negative –
based on the chemical and physical properties of their cell walls. Often the first step in identifying
bacteria.