LaMotte Fresh Water Aquaculture - Manual (Page 7)

7
TEST METHODS
This test kit uses two basic analytical procedures common to fi eld test kits. A brief
explanation of each follows:
COLORIMETRIC: OCTA-SLIDE 2 VIEWER
In a visual colorimetric test, a sample is treated with reagent(s) to produce a color
reaction, generally in proportion to the amount of test factor present. The sample color
is then compared against color standards representing known concentrations of the
factor being tested over a specifi c range.
DILUTIONS
The calibrated test tubes (0106) included in this kit may be used to perform dilutions
for the Ammonia Nitrogen and Nitrite Nitrogen tests. Distilled or deionized water is
needed to perform dilutions.
The following table provides a quick reference guide for dilutions of various
proportions. Once the dilution is prepared, use this diluted sample to perform the test,
and multiply the result by the dilution factor to obtain the actual concentration.
Sample Size Distilled Water to
Bring to 10 mL
Dilution Factor
5.0 mL 5.0 mL 2
2.5 mL 7.5 mL 4
Hold the Octa-Slide 2
Viewer so that non-
direct light enters
through the back of
the viewer.
Insert Octa-Slide 2
Bar into the
Octa-Slide 2
Viewer (1101). Insert test tube
containing reacted
sample into the
Octa-Slide 2
Viewer (1101).
Match sample color
to color standard.
Record results.
1 2 3
4 Note:
If sample color is between two standards, the midpoint
is taken as the result.
If the sample is darker than the highest standard, a
dilution may be performed on a fresh sample, and the
test repeated to bring the concentration within range.