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LaMotte PLANT TISSUE TEST KIT PT-3R User Manual

Page 3

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NI TRATE NI TRO GEN TEST
1.

Use a clean 1 mL pipet (0354) to transfer 1 mL of the filtered tissue
extract to one of the larger depressions on the spot plate (0159).

2.

Use the plastic pipet with screw cap (0392) to add 10 drops of
*Nitrate Reagent #1 (5146) to the filtrate in the spot plate.

3.

Use the 0.5 g spoon (0698) to add 0.5 g of *Nitrate Reagent #2
Powder (5147). Stir thoroughly with a Stirring Rod (0519).

4.

Allow to stand 5 minutes for full color development. Then observe
the color. Compare resulting color from the healthy plant test with
the problem plant test.

Results:

Dark Pink Color

Abundant nitrate

Light Pink Color

Adequate nitrate

No color

No nitrate reserve, probably deficient

PHOS PHO RUS TEST
1.

Fill the “Phosphorus B” Tube (0244) to the line with the filtered
tissue extract.

2.

Use the glass pipet with screw cap (0341) to add 6 drops of
*Phosphorus Reagent #2 (5156) to the tube containing the filtrate.

3.

Add one *Phosphorus Reagent #3 Tablet (5157). Cap and shake
until tablet dissolves.

4.

Immediately note the color. Compare the color development from
healthy and problem plants.

Results:

Deep Blue Color

Abundant phosphorus

Light Blue Color

Adequate phosphorus

Yellow to Colorless

Deficient to
Extremely Deficient phosphorus