LaMotte PLANT TISSUE TEST KIT PT-3R - Manual (Page 2)

*WARNING: Reagents marked with an * are considered to be potential health
hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to
www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax.
To order individual reagents or test kit components, use the specified code number.
Distilled water should be used in the extraction procedure. Natural waters
may be used only if they are free of nitrates (i.e., a nitrate test is performed on
the water sample blank gives no result). Phosphorus and potassium, if present,
are usually present in trace amounts only.
COL LEC TION & PREP A RA TION
OF PLANT TIS SUE SAM PLES
Fresh plant material should be obtained from the growing crop, both from
normal and problem plants. It is important to test both healthy and problem
plants, as the tests are most meaningful when used in a comparative manner.
Select small lots of the leaf petioles, or succulent portion of the stem, in the
part of the plant most affected by any observable abnormal symptoms. Using a
clean, sharp knife or razor blade, cut the material into fine bits of not more
than g to
1
16
inch in length and thickness. An amount of material is used
that will fill the Extraction Tube (0704) to the bottom mark when placed in
the tube without packing.
EX TRAC TION PRO CE DURE
1.
Fill the Extraction Tube (0704) to the lower line with the plant tissue
to be tested.
2. Use a 1 mL pipet (0354) to add 2 mL of *Universal Extracting
Solution, Concentrated (5227).
3. Fill the tube to the upper line with distilled or Nitrate-free water.
4. Cap and shake vigorously for 5 minutes.
5. Use a sheet of filter paper (0465) and the funnel (0459) to filter the
tissue extract into a clean Extraction Tube.