LaMotte DETERGENT TEST KIT DS-1-DC - Manual (Page 2)

Bottom Layer Top Layer Quick Reading
Colorless Colored No Detergent in sample
Some Color Some Color Some Detergent in sample
Colored Colorless High Detergent in sample
NOTE: If the amount of detergent in the sample is to be determined, save
this Test Sample and proceed to Step 2.
STEP 2 - Determine the Amount of Detergent Present
1.
Use the calibrated test tube (0755) to measure 5 mL of detergent-free water.
Add to the screw cap tube marked Reference Sample (0283). (On field trips
it may be necessary to carry a small supply of detergent free water.)
2. Use the 0.25 g spoon (0698) to add one measure of pH Adjustment Powder
(4509). Shake until dissolved.
3. Fill the pipet (0347) with *DS Indicator Reagent (4508) by squeezing the
rubber bulb, then inserting pipet into reagent. Add this amount of *DS
Indicator Reagent to the Reference Sample tube.
4. Add one drop of DS Reference Solution (4513). Cap and shake for one
minute.
5. Allow the tube to stand until the two layers of solution separate. The color
produced in the bottom (water) layer is equivalent to 1 ppm of detergent.
6. Compare the color in the bottom layer of the Test Sample Tube from Part I
to the color of the bottom of the Reference Sample Tube.
If Test Sample Color Is: Test Sample Contains:
Lighter than Reference
Less than 1.0 ppm Detergent
Same as Reference
1.0 ppm Detergent
Darker than Reference
More than 1.0 ppm Detergent
7. Add one drop of DS Reference Solution (4513) to the Reference Sample
Tube. Shake to mix. Compare the color as before. The color in the
Reference Sample is now equal to 2.0 ppm. Continue this procedure,
counting the number of drops of DS Reference Solution (4513) added, until
the color of the bottom layer in each tube is the same. Each drop of the DS
Reference Solution (4513) added to the Reference Sample Tube is equal to 1
ppm detergent in the sample.
NOTE:
If at any time the top layer of the Test Sample or Reference Sample
becomes colorless, add more DS Indicator Reagent (4508). The amount of
this reagent added is not important as long as there is some color in the top
layer.
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