Metrohm 850 Professional IC AnCat - Manual (Page 108)

5.1 Problems and their solutions
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850 Professional IC – AnCat
Problem Cause Remedy
Extreme spread of
the peaks in the
chromatogram.
Splitting (dual
peaks) Connections – dead vol-
ume in the system. Check the connections (see Chapter 2.5, page
14) (use PEEK capillaries with an internal diam-
eter of 0.25 mm between the injection valve
and detector).
Guard column – diminished
performance. ￭ Replace guard column (see Chapter 2.20,
page 58).
Separation column – dead
volume at column head. ￭ Install separation column in reverse flow
direction (if permitted by the information
sheet).
￭ Replace separation column (see "Connect-
ing and rinsing the separation column",
page 61).
Greater rise in the
baseline MSM – reduced capacity. Regenerate the MSM (see Chapter 4.11.3.1,
page 84).
Precision problems -
significant scatter-
ing of the measured
values Sample – Gas bubbles in
the sample. Use sample degasser (see Chapter 2.13, page
40).
Injection valve – sample
loop. Check installation of the sample loop (see
Chapter 2.14.1, page 41).
Sample – Rinsing volume
too low. Prolong the rinsing time (see Chapter 4.8,
page 81).
Injection valve – defective. Request Metrohm Service.
Unexpected change
to the retention
times in the chroma-
tograms Separation column –
diminished separating effi-
ciency. ￭ Regenerate separation column (see Chap-
ter 4.14.4, page 93).
￭ Replace separation column (see "Connect-
ing and rinsing the separation column",
page 61).
Eluent – Gas bubbles in the
eluent. Check connections of the eluent degasser (see
Chapter 2.9, page 31).
High pressure pump –
defective. Request Metrohm Service.
Vacuum is not being
built Eluent Degasser – Connec-
tor Vacuum on the rear of
the instrument not (tightly)
sealed. ￭ Seal the connector Vacuum tightly with a
6.144.6040 threaded stopper.
or when using an 872 Extension Module: