C.B.S. Scientific SG Series - Manual (Page 14)

C. B. S. œ Scientific 14 C.B.S. LITE Slab Gels
4.2 Recommended Buffers and Reagents-continued
BUFFERS:
Agarose Page/Agarose Slab
TBE (1X solution): TAE (1X):
0.089M Tris base 0.04M Tris-acetate
0.89M Boric acid 0.001M EDTA
0.002M EDTA pH 8.0
pH 8.3
Protein Denaturing
Denaturing/Non-Denaturing/
TG-SDS(1X): Nylon Blotting
0.025M Tris base TT (Tris-Taurine( (1X): TT-SDS (1X):
0.192M Glycine 0.1M Tris base 0.1M Tris base
0.1%(w/v) SDS 0.1M Tricine 0.1M Tricine
pH3 0.1%(w/v) SDS
DNA Sequencing
TTE (Tris/Taurine/EDTA)(1X):
1.78M Tris
0.57M Taurine
0.01M EDTA Na2-2H
2
0
4.3 References
1. Hames, B.D., Rickwoood, D. (ed.) (1990). Gel Electrophoresis of Proteins. A
Practical Approach. 2
nd
edn. IRL Press, Oxford. Ch. 1 & 3.
2. Sambrook, J., Fritsch, E.F., Maniatis, T. (1989). Molecular Cloning. A Laboratory
Manual. 2
nd
edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New
York. 18.47-18.61.
3. Ausubel, F.M., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A.,
Struhl, K. (ed) (1993). Current Protocols in Molecular Biology. Vol. 2, Greene
Publishing Associates, Inc. and John Wiley & Sons, Inc., Ch. 10.