C.B.S. Scientific SG Series - Manual (Page 12)

C. B. S. œ Scientific 12 C.B.S. LITE Slab Gels
3.5 Removing the Gel
1. Turn the power supply off and disconnect the leads from the power supply. Remove the
safety cover from the unit, by placing thumbs on white posts next to red & black
connectors, then pushing down while pulling up with fingers under lid. DO NOT pull on
power cords.
2. Drain upper reservoir using a pipette or syringe. Detach side clamps and remove gel
sandwich. Carefully separate, making sure that the gel remains attached to one plate.
Stain and fix according to your preferred method. Dispose of buffer according to your
institutions safety requirements.
3.6 Maximum Well/Comb Volumes
NOTE: To calculate sample well volume expressed in millimeters (mm) of height
divide maximum volume by tooth depth.
TABLE 1---Combs
Overall
Length
13cm
Tooth
width/
mm
Tooth
depth Spacing
between
teeth
mm 0.75mm
thickness
volume per
tooth
microliters
(ul)
1.0mm
thickness
volume per
tooth
microliters
(ul)
1.5mm
thickness
volume per
tooth
microliters
(ul)
2.0mm
thickness
volume per
tooth
microliters
(ul)
3.0mm
thickness
volume per
tooth
microliters
(ul)
1 130.2 19.1 3.2 1865 2486 3729 4966 7458
2 63.5 19.1 3.2 909 1213 1819 2426 3639
3 equal 41.3 19.1 3.2 591 788 1182 1576 2364
2 w/middle
marker
59.8/
4.2
19.1 3.2 856 1142 1713 2284 3426
4 30.1 19.1 3.2 432 576 1151 1152 1728
5 23.5 19.1 3.2 336 449 673 898 1347
6 19.1 19.1 3.2 273 364 546 728 1092
8 13.5 19.1 3.2 193 257 386 514 771
10 10.1 19.1 3.2 145 194 291 388 582
12 7.9 19.1 3.2 113 151 227 302 453
14 6.4 19.1 3.2 91 121 182 242 363
15 5.7 19.1 3.2 82 109 163 218 327
16 5.2 19.1 3.2 74 98 147 196 294
20 4.2 17.7 2.4 55.72 74.3 111.45 148.7 222.9
29 2.9 17.7 1.6 38.5 51.33 77 102.66 154.0
30 2.8 17.7 1.6 37.2 49.6 74.4 99.12 148.8