C.B.S. Scientific MGV-402 - Manual (Page 5)

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SECTION 1
General Information
1.1 Introduction and Features
C.B.S. Scientific offers four different models of MGV Mini-Vertical Systems for performing SDS-
Page, 2-D electrophoresis or agarose gel separations. The dual units, Cat. # MGV-202 (10 x 10cm)
and MGV-402, (8 x 10cm), provide the capability of running two gels simultaneously under identical
buffer conditions and have internal cooling chambers for temperature control. The single units are
MGV-102 (10 x 10cm), and MGV-302 (8 x 10cm). These systems are especially useful for rapid
analysis of samples in short supply. Small format gels also cut down on the use of expensive
reagents needed for gel formation and running buffer.
Gel casting can be accomplished using our convenient and unique Gel Wrap™ or by using our
multiple gel casting chambers. Alternatively, both the single and dual units accommodate ClearP-
AGE™ precast gels. (See Table 1).
With the use of special accessories, two dimensional (2-D) gel electrophoresis can be performed
on our dual mini-vertical system Cat # MGV-202. First dimension I.E.F. tube gels are run on the unit
using Mini-IEF Tube Gel Adapters (described in the catalogue). Buffer surrounds the tubes and is
cooled by the internal cooling chamber for temperature controlled runs. Second dimension separa-
tion is performed by using the standard accessories. A complete description of 2-D protocol is
included when ordering the Mini-I.E.F. Tube Gel Adapters (cat. # MTGA-200K).
Table 1: Features of Vertical Mini-Gel Systems
1.2 Specifications
Constructions:
Buffer chamber, safety cover, core Polycarbonate/Polysulphone
Electrodes Pure Platinum wire .010” diameter
Power cords Silicone rated 7500VDC,
200mA, 65°C
Combs Teflon
Glass plates Soda-lime float glass & Borosilicate
Spacers PVC
Clamps Polypropylene, stainless steel
Safety Certification EN61010-1-1993 (IEC1010-1)
Table 2: Specifications
Cat. # Single or
Dual
Overall Dimensions
W x D x H (cm)
Precast Gels
W x H (cm)
Glass plate size
W x L (cm)
MGV-302 single 14 x 18 x 10 ClearPAGE 10 x 8 10 x 8
MGV-402 double 15 x 18 x 10 ClearPAGE 10 x 8 10 x 8
MGV-102 single 14 x 19.7 x 12.7 ClearPAGE 10 x 10 11.3 x 10 or 10 x 10
MGV-202 double 17 x 19.7 x 12.7 ClearPAGE 10 x 10 11.3 x 10 or 10 x 10
Cat. # Run Time
(minutes)
Buffer Volumes
(ml)
Cooling Compatible
Multi-Caster
MGV-302 25 - 75 110 - GCX-204(K)
MGV-402 25 - 75 205 internal cooling chamber GCX-204(K)
MGV-102 30 - 90 155 - GCC-204(K)
MGV-202 30 - 90 250 internal cooling chamber GCC-204(K)