C.B.S. Scientific MGV-402 - Manual (Page 12)

12
MGV Mini-Vertical Instructions 9-30-13
3.5 Running the Gel
1. Place unit in authorized work area. Remove
safety cover from the assembled unit by
simultaneously pressing down on white
push pins while lifting up on the acrylic
cabochon(s) on the safety cover as shown in
figures 1A and 1B.
Do not remove safety cover by pulling up
on leads!
2. Place glass plate set(s) or precast gel(s) into
unit with smaller or notched plate facing
towards the upper buffer reservoir. If using
a pre-cast gel stored at 4°C, allow to warm to
room temperature.
3. Use small white clamps to attach plate set to
unit, clamping each side to the sandwich. If
using a Dual unit repeat above.
4. Pour freshly prepared buffer in upper and
lower chamber. Using a pipette or syringe,
thoroughly flush out the wells in the glass
plate sandwich with buffer from the upper
chamber. Boil or heat samples and then
place immediately on ice. Load samples.
If outer lanes do not contain sample, it is
recommended that you run standards and/or
fill outer lanes with loading buffer to reduce
smiling and wrap-around effects.
DUAL UNIT
SINGLE UNIT
1A
1B
2
3