C.B.S. Scientific MGV-402 - Manual (Page 13)

13 www.cbsscientific.com
3.5 Running the Gel
1. Place unit in authorized work area. Remove
safety cover from the assembled unit by
simultaneously pressing down on white
push pins while lifting up on the acrylic
cabochon(s) on the safety cover as shown in
figures 1A and 1B.
Do not remove safety cover by pulling up
on leads!
2. Place glass plate set(s) or precast gel(s) into
unit with smaller or notched plate facing
towards the upper buffer reservoir. If using
a pre-cast gel stored at 4°C, allow to warm to
room temperature.
3. Use small white clamps to attach plate set to
unit, clamping each side to the sandwich. If
using a Dual unit repeat above.
4. Pour freshly prepared buffer in upper and
lower chamber. Using a pipette or syringe,
thoroughly flush out the wells in the glass
plate sandwich with buffer from the upper
chamber. Boil or heat samples and then
place immediately on ice. Load samples.
If outer lanes do not contain sample, it is
recommended that you run standards and/or
fill outer lanes with loading buffer to reduce
smiling and wrap-around effects.
5. Attach safety cover as shown in figure 5.
6. Connect the leads to the power supply,
matching the color-coded red to red and
black to black. See Section 4.1 for recom-
mended power conditions. Begin separation
by electrophoresis.
3.6 Removing the Gel
1. Turn the power supply off and disconnect
the leads from the power supply. Remove
the safety cover from the unit as shown in
figures 1A and 1B on page 12.
Do not remove safety cover by pulling up
on leads!
2. Detach side clamps and drain upper buffer
into lower chamber. Remove gel sandwich
and carefully separate making sure that the
gel remains attached to one plate. Stain
and fix according to your preferred method.
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