C.B.S. Scientific DGGEK-2401 - Manual (Page 20)

20
Cipher DGGE Instructions 2/28/14
3 2 Gel Casting Techniques- continued
D Vertical gradient gel casting using GM-40 gradient maker, Mini-Pump and
Multi-Gel Caster
1. Clean the gel caster with soap and water and the glass plates with alcohol.
2. Place a separating sheet in the gel caster.
3. Place the back plate (non-eared) on the sheet.
4. Place the spacers on each side of the back plate.
5. Place the top plate (eared) on the spacers to form 1 st sandwich. Repeat as
required. Continue to Section 3.3 Vertical Gradient Formation.
Fig 3-7