C.B.S. Scientific DGGEK-2401 - Manual (Page 2)

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Cipher DGGE Instructions 2/28/14
T A B L E O F C O N T E N T S
Important User Information 3-4
Section 1: General Information
1 1 Introduction 5
1.2 Specifications 5
1.3 Safety 6
Section 2: DGGEK Unit Set-Up
2.1 Unpacking Instructions 6-7
2.2 Filling DGGEK Tank with Buffer 7
2.3 Heater/Stirrer/By-Pass Pump (Controller) Instructions 8-13
2.4 Setting Buffer Temperature 14
2.5 Connecting Power Supply 14-15
Section 3: Gel Casting Options
3.1 Preparation of Glass Plates 16
3.2 Gel Casting Techniques 16-20
3.3 Vertical Gradient Formation 21
3.4 Perpendicular Gel Casting 22-23
Section 4: Gel Running Preparations
4.1 Preparation of Cassettes 24
4.2 Buffer Cycling Set-Up 25-26
Section 5: Running Conditions
5.1 Running the Gels 26
5.2 Removing the Gels 27
5.3 Buffer Disposal 27
Section 6: Recommended Buffers
6.1 Stock Solutions 28
Section 7: Comb Specifications 29
Section 8: References 30
Section 9: Troubleshooting 31
Section 10: Maintenance of Equipment
10.1 Care and Handling 32
10.2 Maintenance 32
Section 11: Ordering Information 33
Section 12: Regulatory Compliance 34-35