C.B.S. Scientific DGGEK-2401 - Manual (Page 16)

16
Cipher DGGE Instructions 2/28/14
SECTION 3
Gel Casting Options
3 1 Preparation/Cleaning of Glass Plate for Gel Casting
Hand wash both plates with a high quality lab detergent followed by a complete
rinsing with dH
2
O. Air dry or use a lint-free tissue. Spray/wipe the chosen
inner surfaces of the plate set with 95% ethanol and dry with lint-free tissue.
3 2 Gel Casting Techniques
A. Gel Wrap™ Gasket Casting Method.
B. Vertical gradient gel casting using GM-40 gradient maker and gravity flow.
C. Vertical gradient gel casting using GM-40 gradient maker and a Mini-pump.
D. Vertical gradient gel casting using GM-40 gradient maker, Mini-pump and
Multi-gel Caster.
NOTE: Prior to casting, mark the notched glass plate to designate which side is the
‘inside’ and ‘outside’. The same side of the notched plate should always sit against the
reservoir gasket.
A Gel Casting using Gel Wrap™ Gasket Casting method
For Vertical DGGE, use the set of spacers which do not have the small hole or channel
milled into the lower end, these are for casting
perpendicular gels.
1. Start by holding the rectangular back
plate with the rounded bottom corners
and start applying the gasket around one
side of the glass plate. Note: one side
of the “U” shaped gasket is flat, and the
other side has tubing that will act as a
seal around the spacers.
2. When applying the gasket over the
rounded corners of the back glass plate,
make sure the notches on the gasket
align with the rounded corners of the
glass plate. Once the gasket is pushed
over the bottom edge and corners, work
it down the remaining side.