C.B.S. Scientific GCSGU-2640T-02 - Manual (Page 9)

www.cbsscientific.com Horizontal Productivity Packages 9/2011 9
B. Gel Casting in External Gel Caster.
1. Level external gel caster with leveling
screws on base. Push one of the open sides
of gel tray against black gasket.
2. Push down on gel tray until flat against
bottom of tray and so both sides friction fit
against the black gaskets.
3. Place gel comb(s) into desired slot(s) in gel
tray and pour in hot agaorse. When agarose
has cooled place gel tray into Hoizontal Unit.
Remember, current travels from negative
(black cathode) towards positive (red
anode).
3.3 Running the Gel
1. Add enough buffer to fill both reservoirs and overflow the surface of the gel to a depth
of 2-3mm. Gently remove comb(s). Flush out any air bubbles in the wells. Load the
samples into the sample wells.