C.B.S. Scientific WSGE-014 - Manual (Page 9)

Flexicast instructions
version 5/2011
9
RIGHT ANGLE
TUBE FITTINGS
FLOW
PUMP HEAD
PERISTALTIC
STRAIGHT
TUBE FITTINGS
- +
TO CHILLER
SECtIOn 3
Instructions for Use
3 1 Preparing the Electrophoresis Unit
1. Place Flexicast System on a level work surface in an authorized work area. Place the
adjustable height comb assembly in the gel tray. Loosen the white screws holding the
comb to the backing and adjust comb depth (generally 1-2mm). Gently tighten the
adjusting screws and set the comb aside. See page 12 for maximum comb loading
volumes.
3 2 Coolant Circulation (Flexicast Horizontal cat. # WSGE-014 ONLy)
1. A selection of white tubing adapters (right angled and straight) is provided with each
Flexicast WSGE-014 unit. For coolant circulation, screw tubing adapters into fittings
on side of internal cooling chamber, located under the gel bed. Connect a heavy wall
tubing which will not kink (if tubing kinks, excessive pressure build-up may occur
and damage unit) and attach a regulated pump or recirculating water bath (follow
manufacturer’s instructions). Flow rate is not to exceed 3 liters per minute. Do NOT
use tap or house water as it can be subject to large fluctuation in pressure.
3 3 buffer Recirculation (Flexicast Horizontal cat. # WSGE-014 ONLy)
1. Buffer recirculation is recommended for longer runs to maintain uniform pH and ionic
strength and to reduce buffer changes. Unit is shipped with buffer circulation ports
sealed with four white plugs. For cycling buffer, remove plugs and replace with
threaded tubing adapters. Using tubing adapters and pump, cycle buffer from anode
(+) to cathode (-).