C.B.S. Scientific WSGE-014 - Manual (Page 11)

Flexicast instructions
version 5/2011
11
3 5 Running the Gel
1. Add enough buffer to fill both reservoirs and overflow the surface of the gel to a depth
of 2-3mm. Gently remove comb(s). Flush out any air bubbles in the wells. Load
the samples into the sample wells. NOTE: DO NOT FORGET TO LOAD DNA SIZE
STANDARD.
2. Align safety cover over the unit and carefully attach, so not to disturb samples.
3. Connect the leads to the power supply, matching the color-coded red to red and black
to black. See Section 4.1 for recommended power conditions. Begin separation
by electrophoresis.
3 6 Removing the Gel
1. Turn the power supply off and disconnect the leads from the power supply.
2. Remove the safety cover from the unit, by placing thumbs on white posts next to red
& black connectors, then pushing down while pulling up with fingers under lid. DO
NOT pull on power cords.
3. Using the winged portions of the gel tray gently lift the tray from the unit. Always
wear gloves, eye protection and protective clothing if buffer and/or gel
contains Ethidium Bromide. Ethidium Bromide is a powerful mutagen, gloves,
eye protection and protective clothing should always be worn when handling the gel
or buffer solutions. See Material Data Safety Sheets.
4. View separated fragments under UV light, using proper protection for eyes and skin
(see manufacturer’s instructions).