C.B.S. Scientific WSGE-014 - Manual (Page 15)

Flexicast instructions
version 5/2011
15
4 3 References
1. Hames, B.D., Rickwoood, D. (ed.) (19 .9 .0). Gel Electrophoresis of Nucleic Acids.
A Practical Approach. 2nd edn. IRL Press, Oxford. Ch. 2.
2. Sambrook, J., Fritsch, E.F., Maniatis, T. (19 .8 ..9 .). Molecular Cloning. A Laboratory
Manual. 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New
York. Ch 6.
3. Ausubel, F.M., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A. Struhl, K. (ed) (19 .9 .3).
Current Protocols in Molecular Biology. Vol. 1, Greene Publishing Associates, Inc. and John Wiley &
Sons, Inc., Ch. 2.
SECtIOn 5
Maintenance of Equipment
5 1 Care and Handling
The plastic components of the Midi-Horizontal units are fabricated from acrylic and polycarbonate. Electrodes
and connectors are made from pure platinum, stainless steel, and chrome plated brass. As with any laboratory
instrument, adequate care ensures consistent and reliable performance.
After each use, rinse buffer chamber, gel tray and combs with de-ionized water. Wipe dry with a soft cloth or paper
towel, or allow to air dry. Whenever necessary, all components may be washed gently with water and a non-
abrasive detergent, and rinsed and dried as above. Never use abrasive cleaners, glass cleaning sprays or scouring
pads to clean the components, as these will damage the unit and components.
additional precautions:
• Do not autoclave or dry-heat sterilize the apparatus or components.
• Do not expose the apparatus or components to phenol, acetone, benzene, halogenated hydrocarbon
solvents or alcohols.
• Avoid prolonged exposure of the apparatus or components to UV light.
• Do NOT treat with diethylpyrocarbonate (DEPC)-treated water for extended periods at 37°C. A brief rinse
with DEPC-water is sufficient after a thorough wash.
5 2 Maintenance
The following inspection and maintenance procedures will help maintain the safety and reliable performance of the
Midi-Horizontal systems. Replacement parts can be ordered by calling 1-8 ..58 ..-755-49 .59 . or by contacting your local
distributor.
• Banana plugs and power cords should be inspected regularly. If the banana plugs become loose
or do not feel friction tight replace the plugs or power cords.
• Should power cord assemblies (connectors, wire or shrouds) show any signs of wear or damage
(e.g. cracks, nicks, abrasions, or melted insulation), replace them immediately.
• The platinum wire is secured to the banana jack by compression between a stainless washer and
the jack nut. The nut/washer interface should be tight and free of corrosion.