C.B.S. Scientific GCMGU-602T - Manual (Page 9)

9 www.cbsscientific.com
3.4 Gel Casting using External Gel Caster
1. Level external gel caster using white
thumb screws on base. Place gel tray
into External Gel Caster. Ends of gel
tray friction fit onto place. Position the
comb into receptacle slots of gel tray.
Add additional comb if desired.
2. Make agarose solution according to
manufacturer’s instructions. Stains may
be added during this step. Use accord-
ing to manufacturer’s recommenda-
tions.
Use caution when handling hot
agarose solution! Pour molten agarose
solution into gel tray. Allow to cool
uncovered until agarose turn opaque.
NOTE: (Optional storage) Pre-cast gels
can be stored for up to one month if
wrapped in plastic wrap and stored at
4ºC. Remove combs prior to storing.
3. Remove comb and position the tray in
the unit, remembering, current travels
from negative (black cathode) towards
positive (red anode).
Go to Section 3.5
6. Remove comb(s).
7. Remove baffles from their casting posi-
tion.
8.Turn baffles 180º (handles facing in)
and place into the baffle storage slots
provided at either end of the unit near
both terminals.
Go to Section 3.5