C.B.S. Scientific GCMGU-602T - Manual (Page 10)

10
Midi-Horizontal Instructions 9-24-13
3.5 Running the Gel
1. Add enough running buffer to fill both
reservoirs and overflow the surface of the
gel to a depth of 2-3mm. Flush out any air
bubbles in the wells. Stains may be added
during this step. Use according to manu-
facturer’s recommendations.
2. Load the samples into sample wells. Do
not forget to load DNA size standard lad-
ders. For best visibility load samples on
contrasting background color such as red
or black.
Safe stains may be added during this step.
Use according to manufacturer’s recom-
mendations.
3. Place the safety cover on the unit. Con-
nect power leads to power supply, match-
ing the color-coded red to red and black to
black.
See Section 4.1 for recommended
power conditions. Begin separation by
electrophoresis.
3.6 Removing the Gel
1. Turn power supply off and disconnect
the leads from the power supply. Remove
the safety cover from the Midi-Gel unit by
placing thumbs on white posts and pushing
down while pulling up with fingers under lid.
DO NOT PULL ON POWER CORDS!
2. Gently lift the gel tray from the unit. Al-
ways wear gloves, eye protection and
protective clothing if buffer and /or gel
contain Ethidium Bromide. Ethidium Bro-
mide is a powerful mutatgen; gloves, eye
protection and protective clothing should
always be worn when handling the gel or
buffer solutions. View separated fragments
under UV light, using proper protection
for eyes and skin (see manufacturer’s
instructions). Alternatively, Safe Stains may
be used and viewed using blue light or UV
excitation.