C.B.S. Scientific GCMGU-602T - Manual (Page 7)

7 www.cbsscientific.com
SECTION 2
Description of Parts
2.1
Unpacking
Please verify that your Midi-Horizontal Kit comes complete with the
following components:
2.2 Components/Assembly
SECTION 3
Instructions for Use
3.1 Midi-Gel Unit Preparation and Gel Casting
1. Place Midi-Horizontal unit on a level work
surface in an authorized work area. Place
the adjustable height comb assembly over
the gel bed or gel tray. Loosen the white
screws holding the comb to the backing
and adjust comb depth (generally 1mm).
Gently tighten the adjusting screws and
set the comb aside. See Tables on pages
11-12 for comb volumes.
2. Power leads are shipped
separately and must be at-
tached before using your
Midi-Gel. Thread the power
lead ends into the lid recep-
tacles and rotate in clockwise
direction to hand tighten.
3.2 Stain Options
1) Safe Stains
Safe Stains are a less mutatgenic alternative to DNA and RNA visualization than
Ethidium Bromide and do not require exposure to UV light. Follow the Safe Stain
manufacturer’s instructions and recommendations. Safe stains can be added at 4
different stages of electrophoresis:
a) into the agarose gel solution - Sections 3.2.5 and 3.3.2
b) into running buffer - Section 3.4.1
c) into sample loading buffer - Section 3.4.2
d) after electrophoresis - Section 3.5.2
2) Ethidium Bromide (EtBr) Staining
EtBr is a Mutagen. ALWAYS wear gloves. Dispose of solutions in accordance with
the safety regulations of your institution. EtBr can be added at 3 different stages of
electrophoresis:
a) into the agarose gel solution after the solution has been made molten and
the temperature has dropped to 55°C- Sections 3.2.5 and 3.3.2
b) into running buffer - Section 3.4.1
c) after the electrophoresis run in a stain solution. Use at a final concentration
of 0.5-1µg/ml.- Section 3.5.2