3 gel casting in unit using tapered baffles – C.B.S. Scientific GCMGU-602T User Manual
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Midi-Horizontal Instructions 9-24-13
3.3 Gel Casting in Unit using tapered baffles
1. Remove lid by pushing down on white
thumb posts while pulling up lid with
fingers.
DO NOT PULL ON POWER
CORDS!
2. Place gel tray in center of horizontal gel
bed.
3. Remove tapered baffles from storage
position and slide them snugly into the
baffle guides next to the bed,
handles
to the outside. The tapered baffles will
rest friction tight against the gel bed. Do
NOT press the baffles too tightly into the
stops.
4. Position the gel comb, remembering,
current travels from negative (black cath-
ode) towards positive (red anode). Add
additional combs if desired.
5. Make agarose solution according to
manufacturer’s instructions.
Use caution
when handling hot agarose solu-
tion! Pour molten agarose solution into
gel tray. Allow to cool uncovered until
agarose turn opaque. Stains may be
added during this step. Use according to
manufacturer’s recommendations.