Eppendorf Galaxy CO2 Sensor - Manual (Page 13)

Galaxy O2 Sensor Setup — Instructions
13
1.7 Calibrating the O
2
sensor for Galaxy 170 liter and 48 liter 0.1 - 19 % O
2
option
Requirement
•
Nitrogen source capable of supplying clean gas at 5 PSI (0.35 Bar)
•
6 mm bore PVC tubing
The following instructions applies to all incubators equipped with the 0.1 - 19 % O
2
option.
1. Re-reference the O
2
sensor (see Referencing the O
2
sensor for Galaxy 170 liter and 48 liter
incubators on p. 10).
2. Program the incubator for 0 % O
2
.
3. Disable O
2
control.
4. Connect the tubing from the gas supply/regulator to the black plastic flow restrictor fitted at
the end of the long tube attached to the calibration plug.
5. Remove the hydrophobic filter (if installed) from the O
2
sensor.
6. Carefully screw the calibration plug onto the O
2
Sensor (finger tighten only), (see Fig. 1 on
p. 14).
7. Coil the 3 m tube on a shelf to warm the incoming gas, (see Fig. 1 on p. 14).
8. Run the inlet/outlet tubes through the seal and gently close the outer door of the incubator.
9. Ensure that the gas pressure is set to 5 PSI (0.35 Bar), then switch on (N
2
) gas and allow to
flow for 30 - 45 minutes purging all atmospheres from the sensor.
10. View the O
2
sensor offset from the 'ENG' menu.
11. Verify 'O
2
PERCENT' reading is 0.01 %.
If 'O
2
PERCENT' reading is not 0.01 %, adjust the O
2
'ZERO OFFSET' until the correct
reading is obtained.
12. Allow the incubator to stabilize for 10 - 15 minutes and check for drift.
13. Repeat steps 10 - 12 until you are satisfied that the sensor is stable.
14. Press EXIT four times to return to the main menu.
15. Turn off the gas supply and remove calibration plug, (see Fig. 1 on p. 14).
16. Reprogram the CO
2
level (see operating manual) and enable O
2
control (see step 3).
Hint!
•
Perform the following steps to disable O
2
Control:
a Press ‘USER’.
b Select ‘DISABLE’ and press ‘ENTER’.
c Toggle ‘O
2
control’ to ‘OFF’.
d Press ‘ENTER’ to confirm change.
e Select ‘EXIT’ to return to main menu.
Hint!
•
Perform the following steps to view the O
2
sensor offset:
a Press ‘DIAG’, then select ‘ENG’.
b Enter access code (default code 1973), then press ‘ENTER’.
c Select ‘SYSTEM’, then press ‘ENTER’.
d Select ‘O
2
CALIBRATION’, then press ‘ENTER’.
1
Galaxy O
2
sens
or
se
tup