Eppendorf Biomaster 4830 - Manual (Page 9)

Maintenance
Biomaster ® 4830
English (EN)
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9
5 Maintenance
5.1 Testing the volume
You can test the volume of the Biomaster by weighing the pipetted, demineralized water
with a sufficiently sensitive analytical balance:
The following components must have the same temperature:
• demineralized water
• weighing vessel
• pipette
• pipette tips
Weighings should be conducted at 20 °C – 27 °C.
During the measurement the temperature may not fluctuate by more than ±0.5 °C.
Divide the weight of the water by its density (0.9982 mg/μL at 20 °C) to calculate the
volume. This procedure is based on ISO 8655.
Regularly test the precision and accuracy of the Biomaster to avoid dispensing errors. You
will find an SOP (Standard Operation Procedure) for testing pipettes on our website at
www.eppendorf.com.
5.2 Sterilization
You can fully autoclave the Biomaster (at 121 °C, for 20 min). Before doing so, unscrew
and loosen the upper part of the device from its lower part by 2 turns. Make sure that the
Biomaster has completely dried and cooled down before reassembling the device after
autoclaving. The special plastic material of the housing allows a UV light treatment (up to
254 nm).
The Mastertips can also be autoclaved at 121 °C, 20 min. The temperature and time for
autoclaving must be strictly observed. Otherwise, the proper functioning of the device
cannot be guaranteed.
NOTICE! Damage to device from incorrect handling.
 Do not use any additional disinfectants, decontamination agents or sodium
hypochlorite during autoclaving or UV exposure.
 When autoclaving, please ensure that the temperature does not exceed 121 °C.
 Check the suitability of an agent and the manufacturer information about
resistance to chemicals before using a disinfectant or decontamination agents.
Also observe the material of the pipette.