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4 operation, 1 setting the volume, 2 tip take-up – Eppendorf Biomaster 4830 User Manual

Page 8: 3 aspirating liquid, 4 dispensing liquid, 5 ejecting the mastertip, Operation 4.1, Setting the volume, Tip take-up, Aspirating liquid

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Operation
Biomaster

®

4830

English (EN)

Draft

8

4

Operation

4.1

Setting the volume

1. Press and hold the volume catch.
2. Turn the control button to set the volume.
3. Release the volume catch.

The set volume is now secured against unintentional readjustment.

4.2

Tip take-up

Prerequisites
The Mastertip is placed in a tip box.

1. Attach the Biomaster to one Mastertip.

You will feel the Biomaster locking in place in the Mastertip.
Keep the Mastertip in the tip box after attaching it to the Biomaster.

2. Firmly press the control button down to the second stop.

The piston is now fixed to the Biomaster.

3. Release the control button.

The piston will slide back up inside the tip until it reaches its final position.
The system is now ready for operation.

4.3

Aspirating liquid

1. Press down the control button to the first stop.
2. Immerse the Mastertip vertically approx. 3 mm into the liquid.
3. Let the control button slide back slowly.
4. Hold the Mastertip against the inner wall of the vessel while pulling it out of the liquid.

Wipe off any drops on the outside with a lint-free cellulose tissue. Make sure not to
aspirate any liquid from the Mastertip when wiping off the drops.

4.4

Dispensing liquid

1. Hold the Mastertip against the inner wall of the vessel at an angle.
2. Press down the control button to the first stop.
3. Hold down the control button and hold the Mastertip against the vessel wall while

pulling it up.

4.5

Ejecting the Mastertip

 Press down the control button completely to eject the Mastertip.

Small amounts of residual liquid at the tip of the Mastertip are not part of the
dispensing volume and do not have any effect on precision and accuracy.