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Eppendorf Multiporator - Electroporation User Manual
Multiporator
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Eppendorf Multiporator - Electroporation User Manual | 28 pages
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Table of contents
Document Outline
Multiporator – Basic Applications Manual – Electroporation
Contents
1 Introduction
1.1 Purpose of the applications guide
2 Principle of the Multiporator
3 Optimizing the parameters
3.1 Optimizing the field strength
3.2 Length of the field pulse
3.3 Number of field pulses
3.4. Adjustment of the electroporation buffer
3.5 Influence of DNA/RNA
3.6 Influence of the temperature
3.7 Influence of the cell density
4 Electroporation protocol
4.1 Preparing the cells
4.2 Electroporation procedure
4.3 Follow-up treatment of the cells
4.4 Determination of transfection efficiency in the cells
5 Troubleshooting
6 Ordering information
7 Ordering information for North America
8 Appendix
8.1 Guide to determining the minimum voltage to be set on the Multiporator
8.2 Volumes of hypoosmolar and isoosmolar electroporation buffer for setting required osmolarity (10ml)
8.3 Composition of electroporation buffers
8.4 Protocol for the electroporation of eukaryotic cells, based on Jurkat
Eppendorf Offices
4308 900.091-09/0308
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