8 appendix – Eppendorf Multiporator - Electroporation User Manual
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Fig. 4a:
Minimum pulse voltages at which the cell membrane is permeated
The minimum pulse voltage is dependent on the cell diameter following 10- to 15-minute incubation in electroporation
buffer as well as on the temperature and the gap width of the cuvettes. These values, shown as a graph (4a) and as a
table (4b), can be used to determine the optimal pulse voltage to be set on the Multiporator
®
. The optimal pulse voltage
may be 2 to 3 times higher for suspension cells and 2 to 5 times higher for adherent cells.
* The maximum voltage attainable with the eukaryotic module of the Multiporator
®
is 1,200 V.
Fig. 4b
Diameter of cell
[µm]
Voltage
2-mm cuvette
room temp.
Voltage
4-mm cuvette
room temp.
Voltage
2-mm cuvette
4 °C
Voltage
4-mm cuvette
4 °C
5
530
1100
1100*
*
10
270
540
540
1100
15
180
360
360
710
20
130
260
260
530
25
110
220
220
430
30
90
180
180
360
35
80
160
160
310
40
70
140
140
270
45
60
120
120
240
50
50
100
100
210
60
40
80
80
160
80
30
60
60
120
8.1 Guide to determining the minimum voltage to be set on the Multiporator
®
0
2 0 0
4 0 0
6 0 0
8 0 0
1 0 0 0
1 2 0 0
1 4 0 0
0
20
40
60
80
100
4
°C
RT
Cuvette: 2 mm gap width
400 µl volume
V
oltage [V]
Cell diameter [µm]
0
4 0 0
8 0 0
1 2 0 0
1 6 0 0
2 0 0 0
2 4 0 0
2 8 0 0
0
20
40
60
80
100
4
°C
RT
Cuvette: 4 mm gap width
800 µl volume
V
oltage [V]
Cell diameter [µm]
8 Appendix
8 Appendix
Multipor_Appli_E_poration_en.fm Seite 48 Montag, 30. Januar 2006 2:17 14