Eppendorf Multiporator - Electroporation - Manual (Page 25)

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Table 3
The electroporation buffers supplied by Eppendorf are tested for the following important criteria:
• Conductivity
• pH value
• Osmolarity
• Sterility
• Mycoplasma
• Endotoxins
• Pyrogens
• Cytotoxicity
* The purity of myo-Inositol may vary greatly from batch to batch. It must be pure enough to ensure that, at
280 mOsmol/kg in bidistilled water, a conductivity of 10 µS/cm is not exceeded. The conductivity of individual
myo-Inositol batches
should be measured before the buffer is prepared.
Desired osmolarity ml hypoosmolar buffer (ml) ml isoosmolar buffer
90 mOsmol/kg 10 0
150 mOsmol/kg 6.8 3.2
200 mOsmol/kg 4.2 5.8
250 mOsmol/kg 1.6 8.4
280 mOsmol/kg 0 10
Poration (hypoosmolar) Poration (isoosmolar)
Sterile bidistilled water Fill up to 1000 ml Fill up to 1000 ml
KCl 25 mM 25 mM
KH
2
PO
4
0.3 mM 0.3 mM
K
2
HPO
4
0.85 mM 0.85 mM
myo-Inositol * ad 90 mOsmol/kg ad 280 mOsmol/kg
pH value 7.2 ± 0.1 7.2 ± 0.1
Conductivity at 25 °C 3.5 mS/cm ± 10 % 3.5 mS/cm ± 10 %
8.2 Volumes of hypoosmolar and isoosmolar electroporation buffer for setting required osmolarity (10ml)
8.3 Composition of electroporation buffers
8 Appendix
8 Appendix
Multipor_Appli_E_poration_en.fm Seite 49 Montag, 30. Januar 2006 2:17 14