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5 troubleshooting – Eppendorf Multiporator - Electroporation User Manual

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45

Cuvette has been used
several times.

Using an electroporation cuvette several times
may result in a non-homogeneous electrical
field during electroporation. Only new cuvettes
are recommended for important experiments.

Cells were harvested
at different confluencies.

Harvesting cells at different confluencies can
lead to non-reproducible transfection results.
Cells should always be harvested in the
exponential growth phase.

Conductivity of the electropora-
tion buffers varies (such as when
self-prepared buffers are used).

Check the conductivity of self-prepared buffers
on a regular basis using a suitable measuring
device. A defined conductivity is always
guaranteed with Eppendorf electroporation
buffers.

No transfection

Pulse was not injected into
the cell suspension due to poor
contact between cuvette and
Multiporator

®

.

Check that cuvette and the cuvette insert are
correctly inserted in the Multiporator

®

.

Problem

Possible cause

Solution / comments

5 Troubleshooting

5 Troubleshooting

Multipor_Appli_E_poration_en.fm Seite 45 Montag, 30. Januar 2006 2:17 14