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3 loading the rotor, 4 closing the rotor lid, Loading the rotor – Eppendorf 5418 R Centrifuge User Manual

Page 21: Closing the rotor lid

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Operation

Centrifuge 5418 / 5418 R

English (EN)

21

5.2.3

Loading the rotor

To load the rotor, proceed as follows:

1. Check the maximum load (adapter, tube and contents) per rotor bore.

The maximum load is 3.75 g per rotor bore. This information can also be found on the rotor.

2. Load rotors and adapters only with the tubes intended for them.

3. Insert tubes opposite each other in pairs into the rotor bores. For symmetric loading, tubes that face

each other must be the same type and have the same filling quantity.

To keep the weight differences between the filled tubes low, we recommend taring with a balance. This
is gentle on the drive and reduces the operating noises.

5.2.4

Closing the rotor lid

CAUTION! Risk of injury due to asymmetric loading of a rotor.

Load rotors symmetrically with identical tubes.

Only load adapters with suitable tubes.

Always use the same type of tubes (weight, material/density and volume).

Check symmetric loading by balancing the adapters and tubes used with scales.

CAUTION! Risk from damaged or overloaded tubes.

When loading the rotor, observe the safety precautions on dangers as a result of
overloaded or damaged tubes (see

Warnings for intended use on p. 12).

With the rotor FA-45-18-11 centrifugation is also possible without a rotor lid.

Please also note:

• The tube lids must be closed securely.
• Without the rotor lid, the rotor is not aerosol-tight.
• The centrifugation is slightly louder.
• Spin columns must always be centrifuged with a rotor lid.

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