Penn-Century MSA-250-M for Mouse - Manual (Page 7)

7
How to fill the FMJ-250 High Pressure Syringe
Method 1: Fill syringe directly Method 2: Use Air Purge Kit syringe Method 3: Attach Luer Lock
Adapter and any standard needle
A choice of three methods for loading. The full capacity of the FMJ-250 High Pressure Syringe is 250µl. Once
it is filled, it can be used to administer multiple smaller doses, using the volume spacers provided. (See below).
There are three standard methods for filling the device. NEVER load the syringe through the
MicroSprayer®
Aerosolizer. NEVER insert a sharp object or needle into it, as this may scratch or damage the syringe
seal or damage the white Teflon™ tip of the plunger. Depending on the material being used, you may
use any of the following methods.
§ Method 1: Remove the MicroSprayer® Aerosolizer from the syringe and immerse the distal end
of syringe into the solution to be delivered. Pull back on the plunger. Note: If you pull too far,
the plunger will come all the way out.
§ Method 2: Your FMJ-250 High Pressure Syringe comes with an Air Purge Kit that can be used to
eliminate air bubbles that may become trapped inside the syringe. The 1 ml plastic syringe
supplied with the kit can also be used to fill the FMJ-250 Syringe. This syringe comes with a soft
dispensing tip (red) that can be attached to the plastic syringe and inserted into the distal end of
the FMJ-250 Syringe to fill it. The red plastic tip is soft and will not scratch the seal of the FMJ-
250 syringe or the Teflon™ tip of the plunger. If this method is used, remove the plunger of the
FMJ-250 syringe completely, hold it in an almost horizontal position with the distal end slightly
higher, and begin to fill the device from the distal end until the liquid wells up as a bulge or
meniscus at the proximal end. Then, gently insert the plunger into the proximal end. Remove the
air purge syringe and attach the MicroSprayer®.
§ Method 3. You may fill the syringe from a sterile glass vial or other reservoir by attaching a
hypodermic or blunt dosing needle to the FMJ-250 syringe. Note that the tip of the FMJ-250
Syringe does not have a standard luer fitting. If you wish to attach a needle with a standard luer
screw thread to the FMJ-250 Syringe, you must first attach the red portion of the Luer Lock
Adapter that was provided with your equipment to the FMJ-250 Syringe. Then, attach any
standard hypodermic or blunt dosing needle to the black portion of the Luer Lock Adapter. Then,
insert the tip of the needle directly into the test material, or through the septum of a glass drug
container, and pull back on the plunger to fill the syringe fully. Remove both the needle and
adapter after filling. Attach the MicroSprayer® Aerosolizer.