Penn-Century MSA-250-M for Mouse - Manual (Page 3)

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Air bubbles may sometimes form inside the FMJ-250 High Pressure Syringe that will affect
the quality of the aerosol and must be “purged” or eliminated using the Air Purge Kit provided by
Penn-Century, according our instructions.
Correct placement in the trachea is essential. For obtaining the best results for intratracheal use,
the placement of the very tip of the delivery tube is critical. In published studies, optimal lung deposition
from Penn-Century intratracheal aerosol devices is obtained when the very tip is carefully positioned in
the trachea so that the very tip is near to but not touching the carina (first bifurcation) of the
anesthetized animal. If the tip of the MicroSprayer® Aerosolizer is inserted only halfway down the
trachea, it is possible that the aerosol will impact against the walls of the trachea, turn to large droplets
and be coughed or swallowed.
Cautions for intratracheal use in the animal.
Use of all Penn-Century devices for intratracheal
applications is essentially a form of intubation. Familiarity with intubation and anesthetized animal prep is
a prerequisite for use of the devices. When using the any Penn-Century intratracheal aerosol device –
whether liquid or dry powder, the user must push the plunger of the syringe fast and with force to
aerosolize the liquid inside. For optimal lung deposition, the tip of the delivery tube must be positioned
near to, but not touching the carina. Therefore, the user must exercise care and attention to
minimize any forward motion of the hand while pushing quickly and firmly on the plunger, to
prevent trauma or injury to the animal.
Tip of device
Trachea
Aerosol
Carina
Intratracheal use of Penn-Century
devices. For best results, position
the tip of the device near to - but
not touching the carina, or first
bifurcation