Penn-Century MSA-250-M for Mouse - Manual (Page 2)

2
Planning for use – What you need to know
Penn-Century intratracheal aerosol devices have a number of unique attributes that should be considered
in your preparations for conducting research. Prior to use in an
in vivo or in vitro model, users are
responsible for determining the feasibility and safety of administering their particular formulation at the
dose volume, composition and frequency they wish to use. In particular, researchers need to know
§ the effect on their formula when it is aerosolized
§ the ability to aerosolize the same formula repeatedly in the MicroSprayer® Aerosolizer
without clogging it, and
§ the minimum and maximum dose levels that can be safely and effectively administered to the
animal.
For proper use for intratracheal drug administration, the Penn-Century MicroSprayer®
Aerosolizer - Model IA-1C and FMJ-250 Syringe require:
· Knowledge of the process for anesthetizing and intubating an animal
· Time to practice loading the syringe (Three methods are offered below.)
· Time to understand how to attach and use the precision dosing spacers provided with the syringe
· Time to practice use of the MicroSprayer® and FMJ-250 Syringe with water only in the open air to
observe the aerosol and to develop a sense of the rapidity and force you must use to create a
uniform plume of aerosol
· Time to practice using the MicroSprayer® intratracheally in the animal and develop a way to
determine that the very tip of the MicroSprayer® is directly above the carina, without touching the
carina
· Time, if needed, to practice use of the device intratracheally with a colored liquid (dye such as Evans
blue or Methylene blue), fluorescent or radio-opaque contrast material to verify that the very tip of
the MicroSprayer® Aerosolizer has been inserted properly in the trachea to assure optimal deposition
and distribution in the lungs.
· Time to determine the optimal dose range for the particular animal model and drug formulation by
increasing doses in small increments, if the range is not known
· A plan for the method or methods you will use to confirm deposition and distribution of your own
drug or formulation in the lung. This may include use of dye, radio-opaque imaging or contrast
agents, dissection and/or bronchoalveolar lavage.
Important reminders
The Penn-Century MicroSprayer® Aerosolizer Model IA-1C is not a hollow needle, catheter
or cannula. It is a patented, liquid-only, air-free atomizer device. It contains small patented
components in the tip that help generate an aerosol when used with the FMJ-250 High Pressure Syringe.
The passages through these components for the Model IA-1C are about 60 microns in diameter. When it
is used to administer viscous formulations or solutions/suspensions, additional care must be taken to
clean the MicroSprayer® Aerosolizer according to our instructions to prevent agglomeration of suspended
particle material in the tip that can block or clog it and interfere with performance.
The Penn-Century MicroSprayer® Aerosolizer is made of narrow, flexible, stainless steel tubing that
can be easily bent. Care must be taken not to manipulate it or force it into a sharp bend or crease, as this
will damage it beyond repair.