Penn-Century IA-1B-R for Rat - Manual (Page 8)

8
Determining deposition
· Practice with dye or contrast materials: Some users have found it helpful when learning to
correctly use the MicroSprayer® Aerosolizer to practice with radio-opaque or fluorescent
materials or water colored with dye, such as Methylene blue dye or Evans blue dye. (We
strongly discourage using India ink for this purpose, as it may contain carbon particulates or
shellac that can clog the sprayer tip over time.)
· Use of all Penn-Century devices for intratracheal applications is essentially a form of
intubation. The user must insert the tip of the device gently down the trachea of the
anesthetized animal, near to but not touching the carina. Particularly when working with small
animals, some users may wish to practice on several test animals by spraying with dye or a
fluorescent or radio-opaque contrast agent to determine if the tip of the MicroSprayer®
Aerosolizer was correctly positioned, and to determine if the test liquid was well-distributed in
the lungs, or was mostly deposited in the trachea or swallowed. Penn-Century also makes
several helpful accessories, including a Small Animal Laryngoscope and a Mouse Intubation
Platform to help users quickly and easily visualize the epiglottis and correctly insert the tip of
the MicroSprayer® Aerosolizer for optimal results.
Clean and test the MicroSprayer® Aerosolizer with filtered or distilled water after use. Then apply
compressed air to expel any remaining liquid. Attach the protective caps and return to original boxes
for storing.
Care of MicroSprayer® Aerosolizer
The MicroSprayer® Aerosolizer must be cleaned immediately and thoroughly after
use to maintain it in good working condition. It consists of stainless steel and other
chemically resistant components and can be subjected to any standard sterilization or
autoclaving procedure: gas, liquid, wet, dry, heat, cold,
dilute acids, dilute bases, etc. In
addition, the MicroSprayer® Aerosolizer can be heated as high as 500 degrees Fahrenheit. To
avoid damage to the device, if you are uncertain about use of a particular method or material,
PLEASE CONTACT US FIRST.
It is essential to fully clean, test, dry and properly store your device after use to insure that it performs
as it should. Solutions and suspensions used in the device are forced under pressure through patented
atomizing components at the tip of the MicroSprayer® Aerosolizer. Over time, if the device is not fully
flushed out and cleaned after use, there may be a build-up of residual material that hardens and clogs
the tip.