Penn-Century IA-1B-R for Rat - Manual (Page 2)

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Planning for use – What you need to know
Penn-Century intratracheal aerosol devices have a number of unique attributes that should be considered
in your preparations for conducting research. Prior to use in an
in vivo or in vitro model, users are
responsible for determining the feasibility and safety of administering their particular formulation at the
dose volume, composition and frequency they wish to use. In particular, researchers need to know
§ the effect on their formula when it is aerosolized
§ the ability to aerosolize the same formula repeatedly in the MicroSprayer® Aerosolizer
without clogging it, and
§ the minimum and maximum dose levels that can be safely and effectively administered to the
animal.
For proper use for intratracheal drug administration, the Penn-Century MicroSprayer®
Aerosolizer - Model IA-1B requires:
· Knowledge of the process for anesthetizing and intubating an animal
· Time to understand how to attach and use the precision dosing spacers provided with the syringe
· Time to practice use of the MicroSprayer® Aerosolizer with water only in the open air to observe the
aerosol and to develop a sense of the rapidity and force you must use to create a uniform plume of
aerosol
· Time to practice using the MicroSprayer® intratracheally in the animal and develop a way to
determine that the very tip of the MicroSprayer® is directly above the carina, without touching the
carina
· Time, if needed, to practice use of the device intratracheally with a colored liquid (dye such as Evans
blue or Methylene blue), fluorescent or radio-opaque contrast material to verify that the very tip of
the MicroSprayer® has been placed properly to assure optimal lung deposition and distribution
· Time to determine the optimal dose range for the particular animal model and drug formulation by
increasing doses in small increments, if the range is not known
· A plan for the method or methods you will use to confirm deposition and distribution of your own
drug or formulation in the lung. This may include use of dye, radio-opaque imaging or contrast
agents, dissection and/or bronchoalveolar lavage.
Important reminders
The Penn-Century MicroSprayer® Aerosolizer Model IA-1B is not a hollow needle, catheter
or cannula. It is a patented, airfree aerosol generator or atomizer. MicroSprayer® Aerosolizer Model
IA-1B is made of rigid, stainless steel tubing. The tip of the device contains small patented components
that help generate an aerosol when used with the appropriate gas-tight syringe. The passages through
these components in the Model IA-1B are approximately 140 microns in diameter. When using it to
administer viscous formulations or suspensions, additional care must be taken to fully clean the
MicroSprayer® Aerosolizer according to our instructions to prevent agglomeration of material in the tip
that can block or clog it and interfere with performance.
Correct placement in the trachea is essential. For obtaining the best results for intratracheal use,
the placement of the very tip of the delivery tube is critical. In published studies, optimal lung deposition
from Penn-Century intratracheal aerosol devices is obtained when the very tip is carefully positioned in
the trachea so that the very tip is near to but not touching the carina (first bifurcation) of the
anesthetized animal. If the tip of the MicroSprayer® Aerosolizer is inserted only halfway down the
trachea, it is possible that the aerosol will impact against the walls of the trachea, turn to large droplets
and be coughed or swallowed.