Multichannel Systems USB-MEA32-STIM4 Manual - Manual (Page 19)

USB-MEA32-STIM4-System
13
Perfusion
The pMEA carrier is equipped with a glass ring. The ring is 6 mm high, and has an outer diameter
of 24 mm. The ring can be used as perfusion chamber. The USB-MEA32-STIM2-System provides
a PH01 (perfusion cannula) on top of the device. For perfusion you need a peristaltic pump, and
for regulating the temperature of the perfusion medium a temperature controller TC01/02,
connected to the PH01.
Suction
Through the perforation in the pMEA, suction can be applied to the slice from below. The suction
keeps the slice in place and ensures a good electrode to tissue contact without the need for a
weight. The images show a hippocampal slice with and without suction being applied from below.
No suction
Suction applied
Suction can be applied manually with a syringe, or with a controlled vacuum pump, also available
from MCS. Please see chapter "Working with the USB-MEA32-STIM4 Amplifier".