Multichannel Systems USB-MEA32-STIM4 Manual - Manual (Page 16)

USB-MEA32-STIM4 Manual
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4.1.2 Data Acquisition
Electrode raw data are acquired from the special designed perforated MEA and digitized by
the analog / digital converter board that is integrated into the main unit. Recorded signals
are converted in real time into digital data streams at sampling rates of up to 50 kHz per channel.
You will not miss even fast biological signals. Data is transferred to the computer via High Speed
USB 2.0 port.
4.1.3 Stimulus Generator
The internal stimulator generates current controlled pulses only. The maximum output current is
+/-256 μA, the resolution is 13 bit, and the time resolution is 20 μs. Twelve stimulation electrodes
can be connected via software control to the four channels of the internal stimulus generator.
Selection of the stimulation electrodes and programming of the stimulation pulses is done either
with MC_Stimulus II or with the LTP-Director. Please read the respective manuals / helps for
more information.
4.1.4 Perfusion
Perfusion Cannula PH01
A PH01 perfusion cannula is already integrated on top of the USB-MEA32-STIM4-System.
Connected to a TC01/02 temperature controller, it is possible to keep the temperature of the
perfusion medium constant at the desired value. There is no heating element below the MEA
as with the regular MEA amplifiers.
Bath or test solutions can be applied at flow rates from 500 μl to 4.5 ml per minute. The PH01
can be connected to any tubing that fits to the perfusion inlet (luer lock) and outlet (1 mm OD
stainless steel cannula). A titanium wire inserted into the cannula results in a turbulent flow that
ensures that the temperature of the liquid is constant across the flow stream. For setting up a
complete perfusion system, a pump and a perfusion port for aspirating the liquid from the culture
chamber are required. Standard setups for MEA recording use a peristaltic pump or syringe pump,
and a standard metal cannula with beveled tip for aspiration.
The PH01 is equipped with a standard platinum temperature sensor (PT 100). The fluid
temperature is controlled by the connected temperature controller TC01/02. To connect
the perfusion cannula PH01, please read PH01 manual for detailed information.
A cable is attached to the PH01 which should be connected to the system ground. A short
cable with two small crocodile clamps is provided to connect the suction cannula and the PH01,
and thereby also ground the cannula.
Warning: The device must not get in contact with fluids! Spilled liquid can damage or even
completely destroy the electronics of the amplifier! This is eminently important when
using a perfusion system. Take care that the flow rates of the inlet and outlet flow match
so that flooding of the amplifier is efficiently prevented.