Ocean Optics USB2000 - Manual (Page 41)

Appendix B
USB2000 Operating Instructions 36
Appendix B: USB2000-FLG Spectrometer
The USB2000-FLG Spectrometer is preconfigured for use with fluorescence applications from 380-1050 nm. It
contains proprietary Silver AgPlus Mirrors which provide an increase in sensitivity of 10-35% over the standard
USB2000 Spectrometer.
The USB2000-FLG contains custom firmware that allows you to program a variable between a Single Strobe
signal and the commencement of an acquisition. You can enable this mode entirely through software configuration,
and you can easily control the mode via the OOIBase32 interface.
About the USB2000-FLG
On the USB2000-FLG, Ocean Optics has altered the functionality of the External Hardware Trigger function and
reprogrammed the Single Strobe signal function. These alterations of the USB2000 enable you to operate in the
Variable Delay Mode without the presence of an external trigger signal. The USB2000-FLG will simulate the signal
to trigger the appropriate circuitry in the spectrometer.
The table below summarizes the capacity of the USB2000-FLG in Variable Delay Mode:
Parameter Value in Normal Acquisition Mode Value in Variable Delay Mode
Integration time User controlled 5 ms fixed
Strobe delay 0
µs
2 – 511
µs + 3 µs
Jitter < 25 ns < 25 ns
Using the USB2000-FLG
1. Install the OOIBase32 software on your PC.
Note: Ensure that you have installed the OOIBase32 software prior to connecting the USB2000-FLG
Spectrometer to your PC.
2. Connect the USB2000-FLG Spectrometer to the USB port of your PC.
3. Start OOIBase32 and ensure that the spectrometer is acquiring spectra.
4. Verify that the driver version is 4.0.4.9 or later. Open the Help | About option in OOIBase32 to obtain this
information. If the driver meets this requirement, proceed to Step 6. Otherwise, proceed to Step 5.
5. Overwrite (if necessary) the outdated version of the driver with the one included with the USB2000-FLG.
Open Windows Explorer, browse to the OOIBase32 directory (C:\Program Files\Ocean Optics\OOIBase32
is the default installation location) and overwrite the existing ooidrv32.dll with the new file.
6. Enter the desired delay value in the Strobe Freq. box (See Figure B-1 on the following page).
7. Enter Variable Delay Mode by placing the USB2000-FLG in External Hardware Trigger mode. In
OOIBase32, select Spectrum | Configure Data Acquisition | External Trigger, and then select the
Hardware Trigger option. You do not need to apply a trigger signal.
(Continued)