Installation, Attaching fibers for absorbance measurements, Attaching fibers for fluorescence measurements – Ocean Optics CUV-ALL 4-Way User Manual
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CUV-ALL Cuvette Holders Installation Instructions
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043-00000-UV-01-201010
Installation
Follow the installation instructions that correspond to your type of measurement.
Attaching Fibers for Absorbance Measurements
For UV Measurements
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Procedure
1. Attach one end of a SMA-terminated optical fiber to one of the collimating lenses with the UV
label and a red lens cap.
2. Attach the other end of this fiber (the illumination fiber) to a UV light source.
3. Attach another SMA-terminated optical fiber to a collimating lens directly opposite of the first
fiber. This fiber also has a red lens cap.
4. Attach the other end of this fiber to the spectrometer.
For VIS Measurements
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Procedure
1. Attach one end of a SMA-terminated optical fiber to one of the collimating lenses with the
VIS label and a black lens cap.
2. Attach the other end of this fiber (the illumination fiber) to a light source.
3. Attach another SMA-terminated optical fiber to a collimating lens directly opposite of the first
fiber. This fiber also has a black lens cap.
4. Attach the other end of this fiber to the spectrometer.
Attaching Fibers for Fluorescence Measurements
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Procedure
1. Attach one end of a SMA-terminated optical fiber to one of the collimating lenses with the UV
label and a red lens cap.
2. Attach the other end of this fiber to a light source or an excitation source. Typically for
fluorescence measurements, the illumination fiber connects to a UV lens.
3. Attach another SMA-terminated optical fiber to the collimating lens immediately to the left or
right of the first fiber. The two collimators must be positioned at a 90° angle for fluorescence
measurements.
4. Attach the other end of this fiber to the spectrometer. Typically for fluorescence
measurements, the read fiber connects to a VIS lens.