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Installation, Attaching fibers for absorbance measurements, Attaching fibers for fluorescence measurements – Ocean Optics CUV-ALL 4-Way User Manual

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CUV-ALL Cuvette Holders Installation Instructions

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043-00000-UV-01-201010

Installation

Follow the installation instructions that correspond to your type of measurement.

Attaching Fibers for Absorbance Measurements

For UV Measurements

Procedure

1. Attach one end of a SMA-terminated optical fiber to one of the collimating lenses with the UV

label and a red lens cap.

2. Attach the other end of this fiber (the illumination fiber) to a UV light source.

3. Attach another SMA-terminated optical fiber to a collimating lens directly opposite of the first

fiber. This fiber also has a red lens cap.

4. Attach the other end of this fiber to the spectrometer.

For VIS Measurements

Procedure

1. Attach one end of a SMA-terminated optical fiber to one of the collimating lenses with the

VIS label and a black lens cap.

2. Attach the other end of this fiber (the illumination fiber) to a light source.

3. Attach another SMA-terminated optical fiber to a collimating lens directly opposite of the first

fiber. This fiber also has a black lens cap.

4. Attach the other end of this fiber to the spectrometer.

Attaching Fibers for Fluorescence Measurements

Procedure

1. Attach one end of a SMA-terminated optical fiber to one of the collimating lenses with the UV

label and a red lens cap.

2. Attach the other end of this fiber to a light source or an excitation source. Typically for

fluorescence measurements, the illumination fiber connects to a UV lens.

3. Attach another SMA-terminated optical fiber to the collimating lens immediately to the left or

right of the first fiber. The two collimators must be positioned at a 90° angle for fluorescence
measurements.

4. Attach the other end of this fiber to the spectrometer. Typically for fluorescence

measurements, the read fiber connects to a VIS lens.