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5 spinning the sample – Iris Sample Processing StatSpin® Express 2 Primary Tube Centrifuge User Manual

Page 9

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5

Spinning the Sample

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CAUTION - Never operate the Express 2 without the rotor in place.

1.

Ensure the appropriate tube inserts are installed. The Express 2 centrifuge is
provided with four tube inserts for 13 x 75 mm tubes (Product No. SV01). Other sizes
are available (See accessories).

2.

Two or four tubes may be processed at one time. The rotor must be properly
balanced to ensure smooth operation.

3.

Use the same size and style tubes in opposite positions. Balance liquid in tubes to
within 0.5 mL.

CAUTION - Replace balance tube every 10 cycles to prevent possible breakage due
to repeated stress of centrifugation.

4.

Close and latch the centrifuge cover.

5.

Select the appropriate cycle by pressing the cycle selector until the desired LED is
illuminated. (See Table 1)

6.

Press start button.

Table 1: Express 2 Cycle Settings. The operator should experiment with different settings to
achieve optimum performance for specific applications. The following are general guidelines:

Setting

Speed

Time

30s Intended to provide a short spin
to immediately separate the red cells

from the plasma/serum.

8500 rpm

30 seconds

120s Intended to produce good
quality plasma/serum from whole

blood for chemistry or coagulation.

8500 rpm

120 seconds

180s Intended to provide a minimal
particle/cell count and should be
used for specialized applications.

(e.g Lipoclear

®

)

8500 rpm

180 seconds

The normal spin (120 s) can be used for most chemistry and coagulation applications.

The hard spin (180 s) can be used for gel separator tubes. (PST/SST)


NOTE: The Express 2 has no “on-off” switch, and therefore is normally left plugged in and “on”.

Unloading

1. Upon completion of the cycle, the rotor decelerates to a complete stop in 25 seconds and

the latch interlock automatically unlocks.

2. Squeeze the black latch pieces together to open cover.
3. Carefully remove tubes using care not to re-suspend the sediment.