Dako Autostainer - Manual (Page 47)

Problem Possible Cause Course of Action
The probe and
wash/blow head
are not going to the
correct locations. The arm has been
knocked out of
calibration. Abort run and save run log. Reprogram the run and
delete the steps from the Protocol Design screen
that have already been completed. Restart run.
Bent probe tip. Verify that all reagent vial caps have been removed
and slide racks are firmly locked in place in the
down position. Contact a Dako representative.
No Staining Insufficient reagent
in vial. If the system detects insufficient reagent to
complete the current step, you have one minute to
add reagent, after which some slides may be
skipped.
Reagents vials were
not loaded in the
correct locations in
the reagent racks. Check the Reagent Map to verify proper locations.
Check Run Log for “Unable to Locate Reagent”
Skipped Slides message.
Slides were not
loaded in the
correct locations. Check the Load Slide printout to verify proper
locations.
Slides were loaded
with tissue side
down. Orient slides properly.
Programming error. Check the Programming Grid to ensure that the
staining run was programmed correctly.
Slides drying out. Make sure adequate dispense volume that is
suitable for desired incubation times is programmed.
Insure that no extreme environmental conditions are
contributing to the drying of the slides (i.e. direct
sunlight, heating/air conditioning vents, hoods, etc.)
The text of the label
does not begin
along the left edge
of the label and the
text is getting cut
off. The Seymour
printer may need to
be reset. To reset the Seymour Label Printer, turn off the
printer. Hold down the green label feed button on
the top of the printer. Turn on the printer while
holding down the green button and continue holding
the button for three seconds. The labels advance
and the printer will print. Release the feed button.
Press and release the feed button one more time to
advance one label. Perform a test print.
Error occurs during
printing. Labels or ribbon
have run out. Replace ribbon in the printer and the printer will
restart.
Incorrect COM port
selected. Select the correct COM port from the Slide Label
Design screen, see the Initializing section.
Incorrect printer is
selected. Select the correct printer model from Slide Label
Design screen, see the Initializing section.
Autostainer Handbook 39