Hoefer DQ300 - Manual (Page 25)

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4.3 Temperature Considerations
Fluorescence is temperature dependent. As the
temperature of the sample increases, the fluo-
rescence decreases. For greatest accuracy, read
the blank, standard, and samples at the same
temperature.
4.4 Positioning Samples
For low concentration samples, cuvettes often
will give slightly different measurements
depending upon their orientation in the sample
compartment. This is due to defects in the shape
of the cuvette that are not visible to the human
eye. We recommend that the cuvette be marked
at the top and positioned in the sample compart-
ment the same way each time to minimize error.
4.5 Data Quality
The DQ300 is only as accurate as the standards
that are used to calibrate it. It is important to
follow good laboratory practices when preparing
all solutions and reagents including standards,
samples, and blank.