Hoefer PR150 - Manual (Page 5)

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Operating instructions
The parts of the PR150 are shown in Fig 1.
(page 1) The product includes two machined
plastic plates and two silicone gaskets, one slot-
ted and one solid. The unit is assembled and
clamped with four nylon screws.
1
To test a single sample with multiple probes or
antisera, separate the sample on a “preparative” mini
format (8.8 × 8.8 cm) gel. This can be done either
with a full-width comb or simply by applying the
sample directly to the top of a gel cast with no wells.
2
If you are analyzing multiple samples on a Hoefer
miniVE or Hoefer SE260 vertical unit, use the
compatible ten well comb (see ordering information)
to form sample wells in the gel. The spacing of the
teeth of this comb correspond the spacing of the
individual chambers in the PR150.
3
After electrophoresis, transfer the protein or nucleic acid
separated on the gel to an 8.8 × 8.8 cm sheet of an
appropriate membrane. The orientation of the membrane
should be marked with a pencil prior to beginning the
transfer. The gel should not be pre-equilibrated prior to
transfer as it tends to shrink the gel.
4
Open the PR150. Place both the sample plate and
the sealing plate with their machined sides facing
upward on a flat surface.
5
Lay the slotted sealing gasket over the sample
chambers so that the edges of the gasket fit exactly
into the shallow gasket seating lip in the plate.
The gasket slots should be aligned with incubation
chambers in the sample plate. This is most easily
done by holding the slotted gasket loosely by one edge
at the end of the slots. Lower the gasket until the
edge falls into place at the edge of the sample plate.